Yes, but the n2 gas is released at 0psi. There is insufficient pressure to evacuate the air already in the room. Also, the room is not air-tight. Even if you had a large canister of some inert gas at high psi (like n2 at 2200psi), you would only clear some the air out of the room for a very short time.
One way to think about this is if you create a vacuum in a 50 gallon drum, the pressure of the air we breathe will crush the drum that force will constantly be acting on the room to equalize the pressure.